Regulatory Effects of Lycium barbarum Extract and Isolated Scopoletin on Atopic Dermatitis-Like Skin Inflammation

Figure 8

Effect of scopoletin on intracellular signal transductions in TNF-α/IFN-γ-stimulated HaCaT cells. HaCaT cells were pretreated with 5, 10, and 50 μM scopoletin for 1 h before the stimulation. Then, HaCaT cells were stimulated with TNF-α/IFN-γ for 30 min, and whole proteins were extracted using Cell Signaling Technology total protein extraction reagent.