Genotoxicity Evaluation of Metformin in Freshwater Planarian Dugesia japonica by the Comet Assay and RAPD Analysis

Figure 3

Genomic DNAs and RAPD profiles of planarian D. japonica. (a, b) The quality of genomic DNAs isolated from D. japonica treated with 10 mmol/L (a) and 50 mmol/L (b) metformin for 1, 3, and 5 days. M is the 1 kb DNA ladder (10,000, 8000, 6000, 5000, 4000, 3000, 2000, 1500, 1000, and 500 bp from top to bottom). CK is control. (c) Reproducibility of RAPD profiles generated from planarian genomic DNA by primer S75. M is the DL2000 DNA marker (2000, 1000, 750, 500, 250, and 100 bp from top to bottom). (d–f) RAPD profiles of genomic DNAs from D. japonica treated with metformin using primers S64, S84, and S5. 0 is control; 1 and 2 represent 10 mmol/L and 50 mmol/L; a, b, and c represent 1, 3, and 5 days, respectively; M is the DL2000 DNA marker.

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