circZNF91 Promotes the Malignant Phenotype of Chronic Lymphocytic Leukemia Cells by Targeting the miR-1283/WEE1 Axis

Figure 2

Knockdown of circZNF91 inhibits the proliferation of CLL cells and induces apoptosis and G1/S cell cycle arrest. (a) The knockdown efficiency of siRNAs targeting circZNF91 in MEC-1 and HG-3 (Si-NC and si-circZNF91#1, si-circZNF91#2, and si-circZNF91#3). (b) CCK proliferation assay at 0 h, 24 h, 48 h, and 72 h. (c) EdU incorporation assay in cells transfected with si-NC and si-circZNF91#1. (d) Apoptotic assay in cells transfected with si-NC and si-circZNF91#1. (e) Cell cycle analysis in cells transfected with si-NC and si-circZNF91#1. (f) The protein levels (Ki-67, cleaved caspase 3, and CCND1) in MEC-1 and HG-3 cells transfected with si-NC and si-circZNF91#1 were detected by Western blot.

(a)

(b)

(c)

(d)

(e)

(f)