circZNF91 Promotes the Malignant Phenotype of Chronic Lymphocytic Leukemia Cells by Targeting the miR-1283/WEE1 Axis

Figure 3

cirZNF91 interacts with miR-1283 in CLL cells. (a) The subcellular localization of circZNF91 was detected in MEC-1 and HG-3 cells. The expression level of circZNF91 in the nucleus and cytoplasm was detected by RT-qPCR. (b) The binding site of circZNF91 and miR-1283 was predicted by CircInteractome. (c) miR-1283 expression level after the transfection with miR-1283 mimic or miR-NC. (d) A dual-luciferase assay using WT and MUT reporters, in the presence of miR-1283 mimic or miR-NC. (e) RNA pull-down assay using biotin-labeled miR-NC or miR-1283 probe. (f) The enrichment of miR-1283 and cirZNF91 in MEC-1 and HG-3 cells was detected by RIP (RNA immunoprecipitation) assay after incubation with anti-Ago2 or anti-IgG. (g) The expression level of miR-1283 in 20 normal subjects and 45 CLL patients was detected by RT-qPCR. (h) A negative correlation between the expression of circZNF91 and miR-1283 in CLL was detected by Spearman correlation coefficient analysis.

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