[Retracted] Silencing of Long Noncoding RNA HLA Complex P5 (HCP5) Suppresses Glioma Progression through the HCP5-miR-205-Vascular Endothelial Growth Factor A Feedback Loop

Figure 2

HCP5 downregulation inhibited cell viability and migration and mitigated tumor growth. U251 cells were transfected with si-NC, si-HCP5#1, si-HCP5#2, or si-HCP5#3. (a) HCP5 expression was determined by qRT-PCR. (b) Cell viability was assessed by MTS assay. (c) Cell migration was examined by scratch wound-healing assay. (d) Proliferation was determined by EdU incorporation proliferation assay. U251 cells were transfected with sh-NC or sh-HCP5 and then injected into nude mice. (e) Tumors isolated from nude mice. (f) Tumor volume was recorded every 5 days from day 8 after injection. (g) HCP5 expression in primary glioblastoma cells (pGCL) and NHA cells was measured by qRT-PCR. pGCL cells were transfected with si-HCP5#2. (h) HCP5 expression level was analyzed. (i) Cell viability was assessed by MTS assay. (j, k) The migration of U251 cells was examined by scratch wound-healing assay after transfection with si-HCP5#2 and si-NC. And the wound-healing rate was measured. (l) EdU was used to examine the proliferation of pGCL cells. Data are presented as the . ; .

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