[Retracted] Silencing of Long Noncoding RNA HLA Complex P5 (HCP5) Suppresses Glioma Progression through the HCP5-miR-205-Vascular Endothelial Growth Factor A Feedback Loop

Figure 3

HCP5 downregulation repressed cell proliferation via interaction with miR-205. (a) miR-205 expression was assessed by qRT-PCR. U251 cells were transfected with si-NC or si-HCP5#2. Untransfected U251 cells acted as a control. (b) miR-205 expression was determined by qRT-PCR. (c) The binding sites between HCP5 and miR-205 were predicted by IntaRNA 2.0. U251 cells were transfected with NC mimics or miR-205 mimics. Untransfected U251 cells acted as a control. (d) HCP5 expression was assessed by qRT-PCR. (e) Relative luciferase activity was performed by dual-luciferase reporter assay. (f) Cell viability was evaluated by MTS assay in transfected U251 cells. (g) Proliferation was determined by EdU incorporation proliferation assay in transfected U251 cells. Data are presented as the . ; .

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