[Retracted] Silencing of Long Noncoding RNA HLA Complex P5 (HCP5) Suppresses Glioma Progression through the HCP5-miR-205-Vascular Endothelial Growth Factor A Feedback Loop

Figure 4

Silencing of HCP5 repressed GBM proliferation through downregulating VEGF-A. U251 cells were transfected with si-NC or si-HCP5#2. Untransfected U251 cells acted as a control. (a) VEGF-A mRNA expression was determined by qRT-PCR. U251 cells were transfected with sh-NC or sh-HCP5 and then injected into nude mice. (b) The expression levels of HCP5, miR-205, and VEGF-A mRNA in tumor tissues were examined by qRT-PCR. (c) VEGF-A protein levels in U251 cells and tumor tissues were determined by western blot analysis. (d) Cell viability was evaluated by MTS assay in transfected U251 cells. (e) Proliferation was detected by EdU incorporation proliferation assay in transfected U251 cells. U251 cells were transfected with pcDNA3.1-VEGF-A/pcDNA3.1 or cotransfected with sh-HCP5 and pcDNA3.1-VEGF-A/pcDNA3.1 and then injected into nude mice. (f) Tumors isolated from nude mice. (g) Tumor volume was recorded every 5 days from day 8 after injection. Data are presented as the . .

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