[Retracted] DNMT3A Regulates miR-149 DNA Methylation to Activate NOTCH1/Hedgehog Pathway to Promote the Development of Junctional Osteosarcoma

Figure 1

miR-149 was significantly low expressed in osteosarcoma tissues and cells. (a) Differentially expressed microRNA in microRNA expression microarray GSE67268 of osteosarcoma tissues. (b) qRT-PCR detection of miR-149 expression levels in cancer tissues and paired paraneoplastic tissues of 42 osteosarcoma patients. (c–e) Analysis of miR-149 451a expression levels in relation to clinical stage, lymph node metastasis, and differentiation level of osteosarcoma patients. (f) miR-149 expression levels in normal human esophageal epithelial cells HET-1A and osteosarcoma cell lines SAOS-2, osteosarcoma 9706, and MG63. In (b–e), each point represents one sample, and data were analyzed for differences using the paired or unpaired test; , . In (f), data were analyzed for differences using one-way ANOVA and Tukey’s multiple comparison test for analysis of variance on data; .

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