[Retracted] DNMT3A Regulates miR-149 DNA Methylation to Activate NOTCH1/Hedgehog Pathway to Promote the Development of Junctional Osteosarcoma

Figure 2

Exogenous overexpression of miR-149 significantly inhibits the growth and metastasis of osteosarcoma cells in vitro. (a) qRT-PCR to detect the expression level of miR-149 in MG63 and SAOS-2 cells after transfection with mimic control or miR-149 mimic. (b) Plate cloning assay to detect the number of clones formed in MG63 and SAOS-2 cells. The number of clones formed. (c) Caspase-3 kit to detect changes in caspase-3 activity in MG63 and SAOS-2 cells. (d) Flow cytometry to detect the proportion of apoptosis in MG63 and SAOS-2 cells. (e) qRT-PCR to detect the EMT-related factors E-cadherin, ZO-1, vimentin, and N-cadherin mRNA expression levels. (f, g) Transwell assay to detect the migration and invasion ability of MG63 and SAOS-2 cells. Each experiment was repeated three times, and data were presented as mean plus or minus standard deviation, and in (a–g), 2-way ANOVA and Tukey’s multiple comparison test were used to analyze the data for differences, .

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