[Retracted] DNMT3A Regulates miR-149 DNA Methylation to Activate NOTCH1/Hedgehog Pathway to Promote the Development of Junctional Osteosarcoma

Figure 3

miR-149 promoter has significant methylation modification. (a) MSP-qPCR detects the methylation level of miR-149 promoter in cancerous and paraneoplastic tissues of 42 osteosarcoma patients. (b) Correlation between miR-149 and its DNA methylation level in tumor tissues of osteosarcoma patients. (c) MSP-qPCR detects the correlation between miR-149 and its DNA methylation level in normal tissues of osteosarcoma patients. Methylation levels of the promoter of miR-149 in tumor tissues from osteosarcoma patients. (d) Correlation between miR-149 and its DNA methylation levels in tumor tissues from osteosarcoma patients. Each point in CD represents one sample, and the data were tested for significant differences using the paired -test, . In (e, f), each experiment was repeated three times, and the data were presented as mean plus or minus standard deviation, using one-way or 2-way ANOVA and Tukey’s multiple comparison test for analysis of variance on data, , .

(a)

(b)

(c)

(d)