[Retracted] DNMT3A Regulates miR-149 DNA Methylation to Activate NOTCH1/Hedgehog Pathway to Promote the Development of Junctional Osteosarcoma

Figure 4

DNMT3A promotes miR-149 DNA methylation modification. (a) Pearson correlation test to analyze the correlation analysis between miR-149 and DNMT3A expression levels. (b) HPA website to retrieve the staining intensity of DNMT3A in normal esophageal tissues and osteosarcoma tissue samples by immunohistochemistry. (c) ChIP-qPCR to detect DNMT3A binding relationship with miR-149 promoter. (d) The luciferase reporter vector pGL3-enhancer containing miR-149 promoter was constructed, cotransfected into 293T cells with different doses of DNMT3A overexpression plasmids, and the expression level of miR-149 in the cells was detected. In (a), each point represents one sample, and the data were tested for significant differences using paired -test, . In (e, f), each experiment was repeated three times, and the data were presented as mean plus minus standard deviation using one-way or 2-way ANOVA and Tukey’s multiple comparison test for analysis of variance on data; , .

(a)

(b)

(c)

(d)