Ajuba Overexpression Promotes Breast Cancer Chemoresistance and Glucose Uptake through TAZ-GLUT3/Survivin Pathway

Figure 7

Ajuba regulates GLUT3/Survivin through TAZ. (a) RNA-sequencing and gene set expression analysis (GSEA) revealed enrichment for Hippo signaling-related genes in MCF-7 cells with Ajuba overexpression. (b) Western blotting demonstrated that Ajuba overexpression increased the protein levels of GLUT3, Survivin, and TAZ. Ajuba knockdown decreased the protein levels of GLUT3, Survivin, and TAZ. RT-qPCR showed that Ajuba positively regulated GLUT3 and Survivin mRNA in breast cancer cells. (c) TAZ siRNA was cotransfected with Ajuba plasmid in BC cells. TAZ knockdown significantly decreased the level of GLUT3 and Survivin. In TAZ depleted cells, the effects of Ajuba transfection on GLUT3/Survivin were largely abolished. (d) Predicting TEAD4 binding site and matrix using JASPAR database. Chromatin immunoprecipitation (ChIP) assay demonstrated that TEAD4 could bind to the GLUT3 and Survivin promoter regions. .

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