A Novel In Vitro Method to Assess the Microbial Barrier Function of Tissue Adhesives Using Bioluminescence Imaging Technique

Figure 4

Results of bioluminescence measurement converted to CFU/mL. After inoculation of 5 μL E. coli Lux culture with different dilutions on test materials at the indicated time points and sites: from (i) above, (ii) bottom, and (iii) cross section. PU-glue and control: inoculation with pure E. coli Lux. PU-glue (1 : 10) and control (1 : 10): inoculation with 1 : 10 dilution of E. coli Lux. PU-glue (1 : 100) and control (1 : 100): inoculation with 1 : 100 dilution of E. coli Lux. There was no detectable bacterial growth with 1 : 1000 dilution of E. coli Lux.