Optimized Collagen Extraction Process to Obtain High Purity and Large Quantity of Collagen from Human Perirenal Adipose Tissue

Table 1

Amino acid analysis of collagen after decoloring with different H₂O₂ concentration.

Amino acids	0.1% H2O2	0.3% H2O2	0.5% H2O2	1.0% H2O2
Aspartic acid	0.254	0.265	0.287	0.296
Threonine	ND	ND	ND	ND
Serine	0.056	0.056	0.070	0.075
Glutamic acid	0.195	0.213	0.210	0.221
Glycine	0.409	0.478	0.447	0.447
Alanine	0.224	0.266	0.269	0.266
Cystine	1.430	1.461	1.578	1.633
Valine	0.231	0.235	0.248	0.251
Methionine	0.063	0.068	0.074	0.074
Isoleucine	ND	ND	ND	ND
Leucine	ND	ND	ND	ND
Tyrosine	1.409	1.435	1.795	1.577
Phenylalanine	1.302	1.305	1.455	1.483
Lysine	ND	ND	0.016	0.028
Histidine	ND	ND	ND	ND
Arginine	ND	ND	ND	ND
Proline	ND	ND	ND	ND
Total (μg/mL)	5.573	5.784	6.450	6.351

ND: not detected.