Optimized Collagen Extraction Process to Obtain High Purity and Large Quantity of Collagen from Human Perirenal Adipose Tissue

Table 2

Amino acid analysis of collagen after noncollagenous protein removal with different NaOH concentrations.

Amino acids	0.1 M NaOH	0.5 M NaOH	1.0 M NaOH	1.5 M NaOH
Aspartic acid	ND	ND	0.196	0.028
Threonine	ND	ND	ND	ND
Serine	ND	0.045	0.052	0.23
Glutamic acid	ND	ND	0.104	ND
Glycine	ND	0.104	0.315	1.23
Alanine	ND	ND	0.222	0.316
Cystine	0.641	0.653	1.510	2.18
Valine	ND	ND	0.201	0.554
Methionine	ND	ND	0.050	ND
Isoleucine	ND	ND	ND	ND
Leucine	ND	ND	ND	ND
Tyrosine	ND	ND	1.541	ND
Phenylalanine	ND	0.997	1.210	2.006
Lysine	ND	ND	ND	ND
Histidine	ND	ND	ND	ND
Arginine	ND	ND	ND	ND
Proline	ND	ND	ND	ND
Total (μg/mL)	0.641	1.798	5.401	6.546

ND: not detected.