Optimized Collagen Extraction Process to Obtain High Purity and Large Quantity of Collagen from Human Perirenal Adipose Tissue

Table 3

Amino acid analysis of collagen after treatment with different concentration of pepsin for atelocollagen preparation.

Amino acids	0.5% pepsin	1.0% pepsin	1.5% pepsin	2.0% pepsin
Aspartic acid	0.014	0.276	0.051	0.071
Threonine	ND	ND	ND	0.025
Serine	ND	0.084	0.029	0.220
Glutamic acid	ND	0.243	0.031	0.262
Glycine	ND	0.401	0.031	0.026
Alanine	ND	0.274	0.151	ND
Cystine	0.561	1.548	1.007	1.022
Valine	ND	0.288	0.129	0.193
Methionine	ND	0.072	ND	ND
Isoleucine	ND	ND	ND	ND
Leucine	ND	ND	ND	ND
Tyrosine	ND	1.666	ND	ND
Phenylalanine	1.182	1.450	1.005	0.898
Lysine	ND	0.028	0.024	0.031
Histidine	ND	ND	ND	ND
Arginine	ND	ND	ND	ND
Proline	ND	ND	ND	ND
Total(μg/mL)	1.757	6.329	2.458	2.749

ND: not detected.