Optimized Collagen Extraction Process to Obtain High Purity and Large Quantity of Collagen from Human Perirenal Adipose Tissue

Table 4

Amino acid analysis of collagen after hydrolysis with several acidic or basic hydrolyzing agents.

Amino acids	Acetic acid	Lactic acid	NaOH	HCl
Aspartic acid	0.08	0.934	0.022	ND
Threonine	ND	ND	0.08525	ND
Serine	ND	ND	0.59575	ND
Glutamic acid	ND	ND	0.02225	ND
Glycine	ND	ND	1.593	ND
Alanine	ND	ND	0.2985	ND
Cystine	1.892	1.486	0.5255	1.168
Valine	ND	ND	0.27525	ND
Methionine	ND	ND	0.152	ND
Isoleucine	ND	ND	1.31575	ND
Leucine	ND	ND	ND	ND
Tyrosine	ND	ND	1.202	ND
Phenylalanine	1.706	2.63	0.911	1.992
Lysine	ND	ND	0.247	ND
Histidine	ND	ND	ND	ND
Arginine	ND	ND	0.029	ND
Proline	ND	ND	ND	ND
Total (μg/mL)	3.68	5.05	7.274	3.16

ND: not detected.