[Retracted] Identification of RPS7 as the Biomarker of Ferroptosis in Acute Kidney Injury

Figure 7

RPS7 promotes ferroptosis in cisplatin-treated BUMPT cells. (a and b) qRT-PCR confirmed the intervention of RPS7 gene in BUMPT cells. (c and d) MTT assay showed that RPS7 overexpression accentuated, while its gene disruption attenuated, cisplatin-induced BUMPT cell injuries. (e and f) Immunoblot analysis confirmed the intervention of RPS7 gene in BUMPT cells. (g and h) Immunoblot analysis revealed that RPS7 overexpression accelerated, while its gene disruption decelerated, the depletion of FTH1, GPX4, and NCOA4 in cisplatin-treated BUMPT cells. CP: cisplatin; Lv-NC: empty vector lentivirus for RPS7 overexpression; Lv-RPS7: overexpression of RPS7 by lentivirus; shNC: empty vector lentivirus for RPS7 knockdown; shRPS7: knockdown of RPS7 by lentivirus; data are presented as .

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