Identification of Pathogenic Mutations in Primary Microcephaly- (MCPH-) Related Three Genes CENPJ, CASK, and MCPH1 in Consanguineous Pakistani Families

Table 1

Primers sequences used to amplify mutation of MCPH1, CENPJ, and CASK.

ID	Gene	Mutation	Forward	Reverse	Product size
MC-1	MCPH1	c.1254delT; p. Asp419fs	ACCAGGAGATCTATCATGCC	AGAAGTCACGCAACTCGAAG	375
MC-2	CENPJ	c.18delC; p. Ser7fs	GTAGCTCAATGCCCAATTGC	AGAAATGTCCACAGCTGCTC	370
MC-3	CASK	c.1289G>A; p. Arg430His	CCTGCCATAAAAATCCACTC	AGTACAGTCCCTGAAAAGCC	412