Standardized Human Platelet Lysates as Adequate Substitute to Fetal Calf Serum in Endothelial Cell Culture for Tissue Engineering

Figure 1

Morphology of HUVEC grown in medium supplemented with either 20% fetal calf serum (FCS) or with different concentrations of human platelet lysates. HUVEC were seeded in a 12-well plate at a density of cells/well and grown for 72 h in either cell culture media supplemented with either 20% FCS or with human platelet lysates MultiPL’30, MultiPL’100, or with laboratory-prepared human platelet lysate (lp-hPL) in different concentrations. Representative light microscopy images of three independent experiments are shown. Scale bars represent 200 μm.