Standardized Human Platelet Lysates as Adequate Substitute to Fetal Calf Serum in Endothelial Cell Culture for Tissue Engineering

Figure 2

Proliferation of HUVEC cultured in media supplemented with either fetal calf serum (FCS) or with human platelet lysates. HUVEC were seeded in a 96-well plate at a density of cells/well and grown for 72 h in media supplemented with either 20% FCS (controls) or with standardized, commercial human platelet lysates MultiPL’30, MultiPL’100, or with laboratory-prepared human platelet lysate (lp-hPL) at different concentrations. Proliferation was determined by BrdU assay and is shown as percentage of HUVEC grown in medium supplemented with 20% FCS (control). Data are expressed as Experiments were conducted in triplicate and repeated three times.