Standardized Human Platelet Lysates as Adequate Substitute to Fetal Calf Serum in Endothelial Cell Culture for Tissue Engineering

Figure 4

Apoptosis of HUVEC cultured in media supplemented with either fetal calf serum (FCS) or with human platelet lysates. Cell viability was determined by RealTime-Glo™ Annexin V Apoptosis and Necrosis Assay. HUVEC were seeded in a 96-well plate at a density of cells/well and grown for 72 h in media supplemented with either 20% FCS (controls) or with standardized, commercial-available human platelet lysates MultiPL’30, MultiPL’100, or laboratory-prepared human platelet lysate (lp-hPL) at different concentrations. Data are expressed as Experiments were conducted in duplicates and repeated three times. indicates (Student’s -test).