Standardized Human Platelet Lysates as Adequate Substitute to Fetal Calf Serum in Endothelial Cell Culture for Tissue Engineering

Figure 6

Tube formation of HUVEC cultivated in media supplemented with either fetal calf serum (FCS) or with human platelet lysates in different concentrations. HUVEC were seeded on μ-slides with a density of cells/well after the cells were grown for 24 h in culture media supplemented with either 20% FCS or with MultiPL’30, MultiPL’100, or with laboratory-prepared human platelet lysate (lp-hPL). Images of tube-like structures were taken after 4.5 h with fluorescence microscopy after calcein-AM staining. Representative light microscopy images of three independent experiments are shown. Scale bars represent 200 μm.