[Retracted] lncRNA MIR4435-2HG Accelerates the Development of Bladder Cancer through Enhancing IQGAP3 and CDCA5 Expression

<div>miR-2467-3p is sequestered by MIR4435-2HG. (a, b) Subcellular fractionation and FISH assays (<span class="nowrap"><svg height="12.7112pt" id="M23" style="vertical-align:-3.403299pt" version="1.1" viewbox="-0.0498162 -9.3079 116.094 12.7112" width="116.094pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M797 0V26C739 32 732 36 732 103V296C732 394 682 449 605 449C576 449 550 437 529 423C504 407 475 389 446 366C425 418 382 449 334 449C303 449 279 437 253 421C222 403 201 385 180 371V452C135 432 85 419 41 411V388C99 379 102 374 102 310V103C102 38 93 32 27 26V0H238V26C189 32 180 38 180 103V338C210 363 250 390 289 390C351 390 377 348 377 275V103C377 37 368 32 306 26V0H520V26C465 32 456 38 456 101V296C456 314 455 326 453 338C491 369 529 390 565 390C628 390 653 345 653 274V107C653 36 642 32 583 26V0H797Z"></path></g><g transform="matrix(.013,0,0,-0.013,10.608,0)"><path d="M433 39L423 65C413 59 399 54 387 54C370 54 352 69 352 114V299C352 352 342 392 307 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cells. (c) RIP assay examined the enrichment of MIR4435-2HG in Ago2-RISC. (d) The enrichment of predicted 17 miRNAs in the complexes pulled down by biotinylated MIR4435-2HG probe was measured by RNA pull-down assay. (e) RT-qPCR examined the expression of miR-2467-3p in BCa cell lines and SV-HUC-1. (f) The starBase website was applied to predict the binding sites between MIR4435-2HG and miR-2467-3p. (g) Gene overexpression efficiency of miR-2467-3p was verified by RT-qPCR. (h) The physical interaction between MIR4435-2HG and miR-2467-3p was detected by luciferase reporter assay. <span class="nowrap"><svg height="10.7395pt" id="M24" style="vertical-align:-0.6370001pt" version="1.1" viewbox="-0.0498162 -10.1025 50.9184 10.7395" width="50.9184pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M486 158C486 177 478 202 466 220C413 228 386 236 336 262C386 288 413 297 466 304C478 323 486 347 485 366C470 376 444 381 422 380C389 338 368 319 321 288C323 345 329 372 349 422C339 442 322 461 305 470C289 461 271 442 262 422C281 372 287 345 290 288C243 319 222 338 189 380C167 381 142 376 125 366C125 347 133 322 145 304C198 296 225 288 275 262C225 236 198 227 145 220C133 201 125 177 126 158C141 148 167 143 189 144C222 186 243 205 290 236C288 179 282 152 262 102C272 82 289 63 306 54C322 63 340 82 350 102C330 152 324 179 321 236C368 205 390 186 422 144C444 143 470 148 486 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BioMed Research International

fig3

Figure 3

Figure 3: [Retracted] lncRNA MIR4435-2HG Accelerates the Development of Bladder Cancer through Enhancing IQGAP3 and CDCA5 Expression 