[Retracted] lncRNA MIR4435-2HG Accelerates the Development of Bladder Cancer through Enhancing IQGAP3 and CDCA5 Expression

Figure 4

IQGAP3 and CDCA5 are downstream genes of miR-2467-3p. (a) Overlap of Venn diagram exhibited 2 predicted mRNAs. (b) IQGAP3 and CDCA5 levels were detected in miR-2467-3p-overexpressing BCa cells. (c) The predicted binding regions between miR-2467-3p and IQGAP3 or CDCA5. (d) The relationship among MIR4435-2HG, miR-2467-3p, IQGAP3, and CDCA5 was detected by RIP assay. (e) Luciferase activity in the IQGAP3 3UTR-Wt group, IQGAP3 3UTR-Mut group, CDCA5 3UTR-Wt group, and CDCA5 3UTR-Mut group in BCa cells cotransfected with miR-2467-3pmimics was detected. (f) Transfection of miR-2467-3p inhibitor into BCa cells was performed for miR-2467-3p silencing. (g, h) The expression of IQGAP3 and CDCA5 at mRNA level and protein level was detected in different groups. .

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