[Retracted] Activating IL-6/STAT3 Enhances Protein Stability of Proteasome 20S α+β in Colorectal Cancer by miR-1254

Figure 5

miR-1254 targeting PSMD10 promoted protein degradation of proteasome 20S α+β. (a) RT-PCR analysis demonstrates that miR-1254 levels decrease in colorectal cancer lesions relative to the surrounding tissues. (b) The analysis of RT-PCR demonstrates that PSMD10 levels are elevated in CRC lesions relative to surrounding tissues in paired tissue samples. (c) In the tissue sample of TGGA databases, a negative connection between PSMD10 and miR-1254 transcription was found. (d) HCT116 cells were transfected with negative control (NC) and miR-1254 mimics at varying doses (20 μM and 40 μM). qRT-PCR was used to detect miR-1254 expression. (e) Lip2000 was used to transfect HCT116 with negative control (NC) and different concentrations (20 μM and 40 μM) of miR-1254 inhibitor. qRT-PCR was used to detect miR-1254 expression. (f) miR-1254 inhibited the luciferase activity of the PSMD10 3-UTR reporter plasmid by 50.7% in HEK-293 cells. (g) Western blot investigation has shown that PSMD10 expression was modulated following transfection with miR-1254 mimics and inhibitors in HCT116 cells. (h) HCT116 cells were transfected with miR-1254 mimics for 12 h; at that time, they were administered with different doses of cycloheximide (CHX) and harvested at the given time points. PSMD10 and proteasome 20S α+β were immunoblotted from lysates. , , and .

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