Tex264 Binding to SNX27 Regulates Itgα5 Receptor Membrane Recycling and Affects Cell Migration

Figure 2

Identification of SNX27 as a TEX264-binding protein. (a) GST pull-down assay to confirm direct binding of GST-TEX264 with SNX27 in vitro. Rat brain lysates were incubated with GST-TEX264 or GST-coated beads. After washing and elution, western blotting for SNX27 on brain lysates was carried out with an input control (input) performed in parallel. (b, c) Immunoprecipitation (IP) shows interaction of endogenous TEX264 (b) and SNX27 (c) in rat brain tissues. Equal amounts of total lysates were set as the input control. Control IgG was used as the immunoprecipitation control. (d) Immunofluorescence staining to show colocalization of TEX264 with SNX27 in P0 rat brain cortex sections. Blue: DAPI staining of nuclei; green: anti-SNX27 staining; red: anti-TEX264 antibody staining. . (e) Western blotting to detect TEX264 expression in rat brains at different developmental stages. (f) Quantification of TEX264 expression. TEX264 blots were compared with GAPDH levels, and a ratio was determined and then presented as ratio data. Data shown are the of three independent experiments. No significant difference: one-way ANOVA statistics.

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