Tex264 Binding to SNX27 Regulates Itgα5 Receptor Membrane Recycling and Affects Cell Migration

Figure 4

TEX264 knockdown affects HeLa cell migration but not proliferation. (a) HeLa cells were transfected with siTEX264 or scramble siRNA (Scramble) and seeded on 24-well plates for the EdU assay. The proliferation rate was defined as the ratio of EdU-positive cells (red) to DAPI-positive cells (blue). (b) Quantitative data for the EdU assay. Data are the (); nd = no difference. (c) Migration of TEX264-knockdown HeLa cells determined using a wound-healing assay. HeLa cells were transfected with siTEX264 or scramble siRNA (Scramble), seeded on 6-well plates, and cultured to confluency. WT (untransfected HeLa cells). Cell-free gaps were created, and images of the closed gaps were taken at the indicated time point. The wound width was measured at five sites. (d) TEX264 knockdown significantly inhibits HeLa cell migration. , , and compared to the Scramble or WT cells.

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