Rapid Detection of vanA Resistance Gene from E. faecalis Clinical Isolates Using Duplex Loop-Mediated Isothermal Amplification and Triplex PCR Assay

<div>Result of analytical specificity of the LAMP primers. (a) Positive and negative reaction of the LAMP method. Naked eyes visual detection of LAMP reaction with purified DNA of non-E. faecalis. 1: positive control; 2: negative control; 3: E. faecium; 4: E. avium; 5: E. raffinosus; 6: E. psudoavium; 7: E. hirae; 8: E. durans; 9: S. pneumoniae; and 10: S. aureus. (b) PCR product for analytical specificity of primers. Lane 1: 100 bp DNA ladder; lane 2: negative control; and lane 3: positive control with 109 bp product for enterococcus, 195 bp product for vanA gene, and 305 bp product for E. faecalis.</div>

BioMed Research International

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Figure 2

Figure 2: Rapid Detection of vanA Resistance Gene from E. faecalis Clinical Isolates Using Duplex Loop-Mediated Isothermal Amplification and Triplex PCR Assay

Figure 2 | Rapid Detection of vanA Resistance Gene from E. faecalis Clinical Isolates Using Duplex Loop-Mediated Isothermal Amplification and Triplex PCR Assay 