Treatment with Light-Emitting Diodes of Wavelength 863 nm Delays DMBA/TPA-Induced Skin Tumor Formation and Decreases Proinflammatory Cytokine Levels in ICR Mice

Figure 7

IHC analysis of inflammatory cytokine (IL-1β, IL-6) (group I: control, group II: acetone, group III: DMBA, group IV: DMBA+TPA, and group V: DMBA+TPA+863 nm LED). (a) Images of IL-1β and IL-6 expressions among the adjacent skin close to the tumor are shown (first and third rows). And the images in the second and fourth rows show the results extracted using FIJI ImageJ (provided by the National Institutes of Health) software to analyze the expression levels of IL-1β and IL-6. (b) DAB intensities were extracted and quantified. The IL-1β level was higher in groups II, III, and IV compared to the other groups but lower in group V compared to groups I, II, III, and IV. (c) The IL-6 level was higher in group IV compared to group I but somewhat lower in group V compared to groups I and IV; however, statistical significance was lacking. , , and . Error bar: standard deviation.

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