Jinlida Granules Reduce Obesity in db/db Mice by Activating Beige Adipocytes

<div>JLD inhibits the expression of miR-27a to promote the activation of beige adipocytes. (a) Western blot analysis of thermogenic-related protein UCP1 and PGC-1<i>α</i> oxidation-related protein ATP5A and SDHB expression in X9 cells treated with inducer or inducer and JLD. (b) Quantitative measurement of relative protein expression. (c) qRT-PCR analysis of thermogenic and fatty acid oxidation-related gene expression from X9 cells treated with inducer or inducer and JLD. (d) Relative expressions of miR-27a in X9 cells treated with inducer or inducer and JLD at 1, 3, and 5 days. 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transform="matrix(.013,0,0,-0.013,38.365,0)"><path d="M384 0V27C293 34 287 42 287 114V635C232 613 172 594 109 583V559L157 557C201 555 205 550 205 499V114C205 42 199 34 109 27V0H384Z"></path></g></svg> vs. control group. The samples derive from the same experiment, and gels/blots were processed in parallel. Full-length blots/gels are presented in Supplementary Figure <a href="https://static-preview.hindawi.com/articles/bmri/volume-2022/4483009/figures/#supplementary-material-1">S1</a>B. All graphs are done through GraphPad Prism (V.5.01).</div>

BioMed Research International

fig6

Figure 6

Figure 6: Jinlida Granules Reduce Obesity in db/db Mice by Activating Beige Adipocytes 