Mechanism of Traditional Tibetan Medicine Grubthobrildkr Alleviated Gastric Ulcer Induced by Acute Systemic Hypoxia in Rats

Table 1

Compounds identified in aqueous extract of GTB by UHPLC-Q-exactive hybrid quadrupole-orbitrap mass analysis.

No.	tR (min)	MS (m/z)			MS/MS (m/z)	Molecular formula	Identification
		Observed mass (Da)	Calculated mass (Da)	Error (ppm)
1	0.64	144.10191 [M+H]^+	144.10175	-1.11	103.13138, 84.08134, 98.09679, and 70.06587	C7H13NO2	Stachydrine
2	0.73	136.06177 [M+H]^+	136.06165	-0.88	109.05100	C5H5N5	Adenine
3	0.86	152.05669 [M+H]^+	152.05661	-0.53	110.03516, 135.02991, 128.04541, and 107.04948	C5H5N5O	Guanine
4	2.5	149.05971 [M+H]^+	149.05962	-0.60	121.06486, 118.04142, 131.04907, and 103.05457	C9H8O2	Cinnamic acid
5	3.82	153.05462 [M+H]^+	153.05449	-0.85	125.05972, 93.07038, 111.96861, and 129.97884	C8H8O3	Isovanillin
6	4.39	179.03389 [M+H]^+	179.03365	-1.34	151.03896,114.94835, 123.04412, and 133.02834	C9H6O4	Esculetin
7	4.4	179.03389 [M+H]^+	179.03365	-1.34	123.04412, 117.03340	C9H6O4	7,8-Dihydroxycoumarin
8	5.36	137.05971 [M+H]^+	137.05962	-0.66	109.06524	C8H8O2	Anisic aldehyde
9	6.63	167.07027 [M+H]^+	167.07007	-1.20	125.05970, 121.10149, 84.96030, and 110.03656	C9H10O3	Paeonol
10	7.26	342.16998 [M+H]^+	342.16922	-2.22	297.11166, 265.08533, and 237.09053	C20H23NO4	Magnoflorine
11	7.92	328.15433 [M+H]^+	328.15372	-1.86	237.09117, 297.11176, 178.08595, and 163.06247	C19H21NO4	Boldine
12	8.28	342.16998 M^+	342.16934	-1.87	192.10162, 177.07811	C20H24NO4	Phellodendrine
13	8.84	163.03897 [M+H]^+	163.03870	-1.66	107.04945	C9H6O3	7-Hydroxycoumarin
14	9.15	368.11286 [M+H]^+	368.11218	-1.85	307.05954, 277.04910, 249.05411, and 190.08597	C20H17NO6	(+)Bicuculline
15	9.73	354.13360 [M+H]^+	354.13281	-2.23	188.07033, 275.06979, 188.07033, and 149.05962	C20H19NO5	Protopine
16	9.86	322.10738 [M+H]^+	322.10690	-1.49	279.08868, 234.09065, 307.08350, and 250.08571	C19H15NO4	Berberrubine
17	9.95	447.09219 [M+H]^+	447.09177	-0.94	—	C21H18O11	Baicalin
18	10.39	354.16998 [M+H]^+	354.13232	-106.33	336.12201, 320.09183, 190.08597, and 275.06976	C21H23NO4	Dihydropalmatine
19	10.60	370.16490 [M+H]^+	370.16428	-1.67	290.09338, 188.07036	C21H23NO5	Allocryptopine
20	10.96	336.12303 M^+	336.12247	-1.67	278.08099, 292.09616	C20H18NO4	Berberine
21	11.17	354.16998 [M+H]^+	354.16943	-1.55	338.13928, 306.12180, 192.10165, and 165.09084	C21H23NO4	Dehydroglaucine
22	12.22	334.10738 [M+H]^+	334.10669	-2.07	319.08340, 261.07614, 302.07990, and 290.08054	C20H15NO4	Dihydrosanguinarine
23	12.78	237.18491 [M+H]^+	237.18472	-0.80	196.01671, 182.98506	C15H24O2	Curcumol
24	14.00	249.14852 [M+H]^+	249.14781	-2.85	231.13780, 185.13234, 135.08034, and 119.08567	C15H20O3	Micheliolide
25	14.24	301.07066 [M+H]^+	301.07016	-1.66	286.04666, 147.11650, 258.05185, and 229.04871	C16H12O6	Diosmetin
26	14.72	351.2166 [M+H]^-	351.21790	3.70	333.20121, 305.21259, 289.21765, and 183.10057	C20H30O5	Andrographolide
27	15.52	319.22677 [M+H]^+	319.22577	-3.13	273.22053, 255.21010, 301.21591, and 147.11681	C20H30O3	Isosteviol
28	16.00	331.19039 [M+H]^+	331.18976	-1.90	285.18408, 215.10663, 203.10638, and 171.08023	C20H26O4	Carnosol
29	16.12	469.33075 [M+H]^+	469.33124	1.04	95.08595, 299.20111, 119.08565, and 405.31430	C30H44O4	Glabrolide
30	16.14	317.21112 [M+H]^+	317.21118	0.19	281.19016, 131.08543, 299.20016, and 271.20538	C20H28O3	Cafestol
31	16.16	487.34024 [M+H]^+	487.34180	3.20	451.32074, 187.14790, 119.08562, and 201.16367	C30H46O5	Quillaic acid
32	16.66	251.20056 [M+H]^+	251.20020	-1.43	1187.14793, 215.17897, 233.18958, and 95.08595	C16H26O2	Clareolide
33	18.00	293.17583 [M+H]^-	293.17603	0.68	236.10522, 177.09090, 221.15428, and 249.18590	C17H26O4	6-Gingerol
34	19.08	286.14377 [M+H]^+	286.14334	-1.50	201.05440, 143.04912,135.04396, and 115.05444	C17H19NO3	Piperine
35	20.38	233.15361 [M+H]^+	233.15335	-1.12	187.14793, 145.10107	C15H20O2	Atractylenolide II
36	20.28	233.15361 [M+H]^+	233.15334	-1.16	187.14796, 159.11668, 215.14293, and 145.10109	C15H20O2	Isoalantolactone
37	20.84	231.13796 [M+H]^+	231.13757	-1.69	185.13225, 143.08539, 195.11664, and 157.10092	C15H18O2	Dehydrocostus lactone
38	20.84	231.13796 [M+H]^+	231.13757	-1.69	105.07019, 98.03719, 119.08562, and 131.08542	C15H18O2	Lindenenol
39	21.08	303.23186 [M+H]^+	303.23138	-1.58	257.22589, 123.12687, and 147.11668	C20H30O2	Abietic acid
40	21.22	335.22169 [M+H]^+	335.22305	4.06	289.21722, 129.90915, and 275.20213	C20H30O4	Deoxyandrographolide
41	21.27	319.22677 [M+H]^+	319.22650	-0.85	227.14243, 273.22098, 255.21030, and 161.13228	C20H30O3	Steviol
42	22.42	315.19547 [M+H]^+	315.19513	-1.08	303.97168, 145.06465, 187.11153, and 269.18951	C20H26O3	Kahweol
43	23.26	294.20637 [M+H]^+	294.20685	1.63	161.09586, 137.05959, 179.10640, and 203.10635	C17H27NO3	Nonivamide
44	29.25	279.23186 [M+H]^+	279.23145	-1.47	95.08595, 81.07049, and 67.05501	C18H30O2	Alpha-linolenic acid

t_R: retention time.