T1AM Attenuates the Hypoxia/Reoxygenation-Induced Necroptosis of H9C2 Cardiomyocytes via RIPK1/RIPK3 Pathway

Figure 1

T1AM protects H/R-induced H9C2 cells injury. (a) The effects of T1AM on H/R-induced cell morphology. (b) CCK8 assay () revealed that T1AM improved H/R-induced H9C2 cell viability. (c) LDH release () in the culture medium of H9C2 cells exposed to hypoxia 24 h and reoxygenation 4 h treated with 6 μM T1AM. (d) TUNEL stain positivity and Hoechst stain condensed nuclei of H9C2 cells (). (e) Quantification of TUNEL-positive cells. Control: H9C2 cells cultured in normoxia; T1AM: H9C2 cells cultured in normoxia treated with 6 μM T1AM; H/R: H9C2 cells exposed to hypoxia 24 h and reoxygenation 4 h; H/R+T1AM: H9C2 cells exposed to hypoxia 24 h and reoxygenation 4 h treated with 6 μM T1AM.

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