T1AM Attenuates the Hypoxia/Reoxygenation-Induced Necroptosis of H9C2 Cardiomyocytes via RIPK1/RIPK3 Pathway

Figure 4

T1AM preconditioning alleviates cardiomyocytes necroptosis by the RIPK1/RIPK3 pathway which can be rescued by TNF-α. (a) LDH release. (b) Western blot. (c) The band intensities are quantified. H/R: H9C2 cells exposed to hypoxia 24 h and reoxygenation 4 h; H/R+T1AM: H9C2 cells exposed to hypoxia 24 h and reoxygenation 4 h treated with 6 μM T1AM; H/R+T1AM+TNF-α: H9C2 cells exposed to hypoxia 24 h and reoxygenation 4 h treated with 6 μM T1AM and 20 ng/ml TNF-α.

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