Sinomenine Inhibits Vasculogenic Mimicry and Migration of Breast Cancer Side Population Cells via Regulating miR-340-5p/SIAH2 Axis

<div>Sinomenine upregulates miR-340-5p to repress SIAH2/HIF-1<i>α</i> axis in breast cancer SP cells. (a) Bioinformatics analysis showed the predicted binding sites between miR-340-5p and SIAH2. (b) Breast cancer SP cells were transfected with miR-NC or miR-340-5p mimics, and cultured with 0.75 mM of sinomenine under normoxic or hypoxia, the level of miR-340-5p was assessed by RT-PCR. (c) HEK293T cells were cotransfected with miR-340-5p mimics or miR-NC and SIAH2-wt or SIAH2-mut, the relative luciferase activity was assessed by the dual-luciferase reporter gene assay. (d) The mRNA level of SIAH2 was evaluated by RT-PCR. (e) The protein levels of SIAH2 and HIF-1<i>α</i> was assessed by western blot. 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BioMed Research International

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Figure 3

Figure 3: Sinomenine Inhibits Vasculogenic Mimicry and Migration of Breast Cancer Side Population Cells via Regulating miR-340-5p/SIAH2 Axis 