Subcellular Proteome Analysis Reveals Apoptotic Vulnerability of T-Cell Acute Lymphoblastic Leukemia

Figure 3

Enhanced respiration and energy metabolism in cells resistant to DR5-induced apoptosis. (a) Venn diagram analysis on total number of overlapped proteins identified in Jurkat and JurkatR after pooling data from four subcellular fractions. (b) Upper part: unsupervised hierarchical clustering of all quantified proteins by label-free MS from Jurkat and JurkatR. Lower part: the average intensities distribution of identified proteins from two cell lines. Data were log2 transformed and significance was assessed using Mann-Whitney (unpaired and nonparametric) test. (c) Most significantly regulated proteins in JurkatR. The -axis: ratio of protein intensity in JurkatR over the corresponding protein intensity in Jurkat. Red: (upregulated); blue: (downregulated). (d) Upper part: unsupervised hierarchical clustering of proteins identified in indicated subcellular fractions from Jurkat and JurkatR. Lower part: Venn diagram showed the overlaps of proteins identified in the corresponding subcellular fractions. (e) GO analysis (cellular component) on proteins identified in NP fractions from Jurkat and JurkatR. The -axis: enrichment ratios were log2 transformed. The -axis: enrichment –log10 of significant value.

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