[Retracted] LINC00707 Promotes Cell Proliferation in Cervical Cancer via the miR-374c-5p/SDC4 Axis

Figure 3

SDC4 is the target gene of miR-374c-5p in CC cells. (a) Potential mRNAs that may bind to miR-374c-5p were selected using starBase. (b) RNA pull-down assay was taken to measure the enrichment of selected two mRNAs (SDC4 and ARID4A) in Bio-miR-374c-5p-WT/Mut groups. (c) The identified SDC4 expression in CC cell lines. (d) RIP assay was performed to test the enrichment of LINC00707, miR-374c-5p, and SDC4 in the anti-Ago2 group and the control IgG group. (e) miR-374c-5p expression was elevated in the SiHa cell line via miR-374c-5p mimic transfection while it was reduced via miR-374c-5p inhibitor transfection in C-33A cells. (f) The relative expression along with protein level of SDC4 upon miR-374c-5p overexpression or inhibition. (g) The binding sites between miR-374c-5p and SDC4 were evidenced by the luciferase reporter assay. (h) SDC4 expression in sh-LINC00707#1-transfected CC cells. Error bars represent the of at least three independent experiments. , , and .

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