[Retracted] Berberine Attenuates IL-1β-Induced Damage of Nucleus Pulposus Cells via Activating the AMPK/mTOR/Ulk1 Pathway

Figure 1

Berberine accelerates cell proliferation and suppressed apoptosis in IL-1β-induced NP cells. (a) NP cells were treated with 0, 0.13, 0.25, 0.50, 1.00, 2.00, 4.00, 8.00, and 16.00 μM BBR for 7 days; then cell viability was detected by CCK-8 assay. (b) NP cells were treated with IL-1β in combination with 0, 0.25, 0.50, 1.00, 2.00, and 4.00 μM of BBR; then cell viability was measured by CCK-8 on 0, 2, 4, and 6 days posttreatment. (c) NP cells were treated with 1.00 μM BBR, IL-1β, or 1.00 μM BBR combined with IL-1β, and cell viability was measured by CCK-8 on 0, 2, 4, and 6 days posttreatment. (d) NP cells were stained with BrdU and DAPI. (e) Relative BrdU-positive cells were counted and presented. (f) NP cells were treated with BBR alone or combined with IL-1β, and apoptosis were detected by FACS. (g) Percentage of apoptotic cells was presented. (h) NP cells were treated with BBR alone or combined with IL-1β, and protein expressions were analyzed by immunoblot. (i) Relative protein expression was analyzed by the ImageJ software. Scale bars: 25 μm, magnified ×400. The data are shown as the of three experiments. compared with the IL-1β induced group; compared with the control group; compared with the IL-1β induced group.

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