Research Article
[Retracted] Berberine Attenuates IL-1β-Induced Damage of Nucleus Pulposus Cells via Activating the AMPK/mTOR/Ulk1 Pathway
Figure 2
Effect of Berberine on ECM-related protein expression and ECM degradation in IL-1β-induced NP cells. (a) NP cells were treated with 1.00 μM BBR, IL-1β, or 1.00 μM BBR combined with IL-1β, and the mRNA level of aggrecan, collagen II, and MMP-13 was evaluated by RT-qPCR. (b) Confocal imaging of NP cells was collected by staining collagen II antibody (green). (c) Protein levels of aggrecan, collagen II, and MMP-13 were evaluated by immunoblot. Scale bars: 25 μm, magnified ×400. The data are shown as the of three experiments. compared with control group; compared with the IL-1β induced group.
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