Nicotinic Acid Riboside Regulates Nrf-2/P62-Related Oxidative Stress and Autophagy to Attenuate Doxorubicin-Induced Cardiomyocyte Injury

Figure 4

NAR and Dox administration alone or in combination on the cell apoptosis in cardiomyocytes. (a) Flow cytometric dot plots (-axis: Annexin V-FITC staining, -axis: PI staining). (b) The H9c2 cells were analyzed for apoptosis using the TUNEL staining method. The panels display representative histological images (magnification×40).  μm. (c) Quantitative analysis of the fluorescence intensity. (d) Expression of cardiac apoptosis proteins in different treatment groups. Densitometric analysis of (e) caspase-3; (f) Bcl-2; and (g) Bax. The figure shows a representative blot for that protein. The values were expressed as fold changes over the level of β-tubulin, which served as a loading control. The results were analyzed using one-way ANOVA and Student -tests. Data are presented as the . Significance is indicated as . Versus the control, ^#; versus the Dox group, .

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