hnRNPC Promotes Malignancy in Pancreatic Cancer through Stabilization of IQGAP3

Figure 2

hnRNPC promotes PDAC cell proliferation, migration, and invasion. (a, b) Relative hnRNPC mRNA and protein expression in PANC-1, MIApaca-1, SW1990, and BxPC-3 human pancreatic cells and normal cells (HPNE) by RT-qPCR (; , , vs. HPNE) (a) and Western blot (b). (c) The expression of hnRNPC protein by qRT-PCR in BxPC-3 cells which were stably knockdown hnRNPC and PANC-1 cells with stable hnRNPC overexpression. (d) Colony formation assays were performed on BxPC-3 cells which were transfected with scr/sh-hnRNPCRNA#1/sh-hnRNPCRNA#2 and on PANC-1 cells transfected with hnRNPC overexpression vector/empty vector (, , ). (e) MTT assay was performed on BxPC-3 cells which were transfected with scr/sh-hnRNPCRNA#1/sh-hnRNPCRNA#2 and on PANC-1 cells transfected with hnRNPC overexpression vector/empty vector to assess cell growth. (f) Cell migration determined by % area wound healed on BxPC-3 cells which were transfected with scr/sh-hnRNPCRNA#1/sh-hnRNPCRNA#2 and cell migration and invasion determined by a Transwell assay on BxPC-3 cells. (g) Cell migration determined by % area wound healed on PANC-1 cells transfected with hnRNPC overexpression vector/empty vector and cell migration and invasion determined by a Transwell assay on PANC-1 cells. Statistical significance was calculated using one-way ANOVA and Student’s -tests, respectively. All data shown are the of three independent experiments. , , .

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