hnRNPC Promotes Malignancy in Pancreatic Cancer through Stabilization of IQGAP3

Figure 3

hnRNPC interacts with IQGAP3 and promotes its expression. (a) catRAPID fragment-based prediction analysis of interaction between IQGAP3 and hnRNPC identified a strong interaction with high confidence levels ( and ). (b, c) Western blotting analysis of the specific association between hnRNPC and IQGAP3 from RNA pull downs, incubated with lysed cell BxPC-3 (b) and cell PANC-1 (c) supernatant. GAPDH was used as a control. (d) Fold enrichment of IQGAP3 expression in RIP assays performed with hnRNPC coated beads. (e) Relative expression of hnRNPC in PDAC tissues () and adjacent healthy tissues () determined by qRT-PCR. (f) Relative expression of IQGAP3 determined in PDAC cell lines PANC-1, MIApaca-1, SW1990, and BxPC-3, compared to HPNE using qRT-PCR and normalized to GAPDH expression. (g) Expression levels of IQGAP3 determined in BxPC-3 and PANC-1 cells expressing sh-hnRNPC or hnRNPC overexpression, respectively. All data shown are the of three independent experiments. Statistical significance was calculated using Student’s -test or one-way ANOVA. , .

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