[Retracted] Albiflorin Alleviates Ox-LDL-Induced Human Umbilical Vein Endothelial Cell Injury through IRAK1/TAK1 Pathway

Figure 1

AF memorably induced viability and cholesterol efflux and repressed inflammation, ROS production, and apoptosis in Ox-LDL-induced HUVECs. (a) After 0, 4, 20, 100, 200, and 300 μM of AF stimulation, the toxicity of AF was monitored using CCK-8 in HUVECs by calculating the inhibition rate; , , and compared with the 0 μM AF group. (b) After 4 μM AF (AF-L) and 100 μM AF (AF-H) treatment, cell viability was confirmed by CCK-8 in 100 mg/L Ox-LDL-induced HUVECs; compared with the control group and ^# compared with the Ox-LDL group. (c) Percentage of cholesterol efflux (i.e., the proportion of labelled cholesterol moved from cells to the specified acceptor) was tested using the corresponding kit in Ox-LDL-induced HUVECs after 4 μM AF(AF-L) and 100 μM AF(AF-H) treatment; compared with the control group and ^# compared with the Ox-LDL group. (d) The levels of IL-6 and TNF-α were verified by ELISA kits in Ox-LDL-induced HUVECs after AF exposure; and compared with the control group; ^# and ^## compared with the Ox-LDL group. (e) The influence of low- and high-dose AF on ROS production was confirmed using flow cytometry in Ox-LDL-induced HUVECs; compared with the control group and ^# compared with the Ox-LDL group. (f) The impact of AF on cell apoptosis was identified by flow cytometry in Ox-LDL-induced HUVECs; compared with the control group and ^# compared with the Ox-LDL group.

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