Single Nucleotide Polymorphism in the ADIPOQ Gene Modifies Adiponectin Levels and Glycemic Control in Type Two Diabetes Mellitus Patients
Table 1
ADIPOQ SNPs information.
SNP ID
Location and base change
Forward primer reverse primer
PCR program (34 cycles)
PCR amplicon size (bp)
Restriction enzyme, incubation temperature, and time
RFLP product (BP)
rs17300539
Promoter region (A/G)
CAGGAAGCTGAGGCTTTACA ACCCACTTAGGTGTTCCTAGA
95°C 30 sec, 58.5°C 30 s, 72°C 1 min
342
Alu I 37°C, 1 h
AA: 236, 106 AG: 342, 236, 106 GG: 342
rs266729
Promoter region (C/G)
ACTGTGGAGATGATATCTGG CATTTTGACAGCTACCTTGG
95°C 30 sec, 58.5°C 30 s, 72°C 1 min
412
Hha1 37°C, 1 h
GG: 170, 243 CG: 170, 243, 412 CC: 412
rs1501299
Intron 2 (G/T)
TGACCAGGAAACCACGACTC CCATCTACACTCATCCTTGG
95°C 30 sec, 58°C 30 s, 72°C 1 min
341
BsmI 65°C, 1 h
GG: 229, 112 GT: 341, 229, 112 TT: 341
rs2241766
Exon 2 (T/G)
AGTAGACTCTGCTGAGATGG ACATTCTTACCTGGATCTCC
95°C 30 sec, 59°C 30 s, 72°C 1 min
333
BspH1 37°C, 1 h
TT: 153, 180 TG: 333, 153, 180 GG: 333
Abbreviations: SNP: single nucleotide polymorphism; PCR: polymerase chain reaction; RFLP: restriction fragment length polymorphism. All SNP information was obtained from the NCBI dbSNP database.
