[Retracted] hsa_circ_0084811 Regulates Cell Proliferation and Apoptosis in Retinoblastoma through miR-18a-5p/miR-18b-5p/E2F5 Axis

Figure 5

circ_0084811 sponged miR-18a-5p and miR-18b-5p to regulate E2F5 expression. (a) Potential miRNAs of circ_0084811 and E2F5 forecasted through starBase. (b) miR-654-3p, miR-18a-5p ,and miR-18b-5p enrichment in bio-circ_0084811 probe was confirmed via RNA pull-down assay. (c) Predicted binding sites between circ_0084811 and miR-18a-5p or miR-18b-5p, E2F5, and miR-18a-5p or miR-18b-5p. (d) The relationship among circ_0084811, miR-18a-5p, miR-18b-5p, and E2F5 was confirmed through RIP assay. (e) miR-18a-5p mimics and miR-18b-5p mimics were utilized to elevate their expression in RB cells. (f) The affinity of miR-18a-5p or miR-18b-5p with circ_0084811 or E2F5 was testified by luciferase reporter assays. (g) miR-18a-5p and miR-18b-5p were, respectively, silenced in RB cells by inhibitor transfection. (h, i) E2F5 expression in RB cells transfected with sh-NC, sh-circ_0084811#1, sh-circ_0084811#1+miR-18a-5p inhibitor, and sh-circ_0084811#1+miR-18b-5p inhibitor plasmids. Each assay went through three biological replicates. The sample . and .

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