[Retracted] Brusatol Inhibits Proliferation and Metastasis of Colorectal Cancer by Targeting and Reversing the RhoA/ROCK1 Pathway

Figure 4

BRU downregulates RhoA/ROCK1 pathway protein expressions in colorectal cancer cells. After the respective treatment concentrations were added into HCT-116 and SW480 cells, the expressions of RhoA and ROCK1 in the treated and the untreated groups were detected by western blot (a, b), and the results of quantitative analysis by the Image Lab software (c, d). Representative immunofluorescence images of HCT-116 cells treated with or without 6 nM BRU for 24 h (e, f). The mRNA expressions of RhoA and ROCK1 in HCT-116 cells were determined by RT-QPCR (g). All data were shown as from three independent trials (, vs. control).

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