Research Article
A Novel Inhibitor INF 39 Promotes Osteogenesis via Blocking the NLRP3/IL-1β Axis
Figure 1
INF 39 increases osteoblast activity. (a) The structure of INF 39. (b) Cell viability of INF 39-treated mouse calvarial osteoblasts tested by CCK-8 assay at 48 and 96 hours. (c) The ALP activity of mouse calvarial osteoblasts, ALP staining, quantification in cell culture, and cell with various concentrations of INF 39. (d) ALP staining, quantification in cell culture, and cell after knockdown of NLRP3. (e) Alizarin Red S staining and OD values of 405 nm obtained for mineralized matrix solutions following the treatment with INF 39. (f) Alizarin Red S staining and OD values obtained for mineralized matrix solutions after knockdown of NLRP3. Data are shown as the . , , and , compared with the controls.
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