A Novel Inhibitor INF 39 Promotes Osteogenesis via Blocking the NLRP3/IL-1β Axis

Figure 2

Repressing NLRP3 promotes osteoblast-related gene expression. (a–d) Expression of the osteoblast-specific genes Runx2, COL1a, ALP, and OCN in mouse calvarial osteoblasts treated with various concentrations of INF 39 (). Runx2: runt-related transcription factor 2; COL1a: collagen type 1α; ALP: alkaline phosphatase; OCN: osteocalcin. (e) The gray level of RUNX2 was quantified and normalized to β-tubulin using ImageJ (). (f) Expression of NLRP3 mRNA after knockdown of INF 39 (). (g) Expression of the osteoblast-specific genes Runx2 and OCN in mouse calvarial osteoblasts after knockdown of NLRP3 (). (h) The gray level of Runx2 and NLRP3 was quantified and normalized to GAPDH using ImageJ (). Data are shown as the . , , and , compared with the controls.

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