[Retracted] Fluoride Exposure Suppresses Proliferation and Enhances Endoplasmic Reticulum Stress and Apoptosis Pathways in Hepatocytes by Downregulating Sirtuin-1

Figure 3

Fluoride exposure suppresses proliferation, downregulates Sirt-1, and enhances ERS and apoptotic pathways in AML12 cells. AML12 cells were first processed with 0, 10, 25, 50, 75, and 100 mg/L NaF for 36 h. (a) Cell proliferation was determined by CCK-8. (b, c) PERK and Sirt-1 expressions were assessed through RT-qPCR. (d) Western blot was adopted to confirm the protein expressions of PERK, Sirt-1, GRP-78, ATF6, CHOP, and caspase-3. (e) The above six proteins were quantified separately in line with grayscale values. , , .

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