Research Article
[Retracted] Fluoride Exposure Suppresses Proliferation and Enhances Endoplasmic Reticulum Stress and Apoptosis Pathways in Hepatocytes by Downregulating Sirtuin-1
Figure 4
Sirt-1 knockdown enhances suppression of proliferation and induction of ERS and apoptosis mediated by fluorine exposure in AML12 cells. AML12 cells were addressed with 50 mg/L NaF and transfected with Sirt-1 siRNAs. (a) RT-qPCR exhibited the change in the Sirt-1 expression. (b) CCK-8 was utilized to confirm the change of cell proliferation. (c) Western blotting analysis of PERK, Sirt-1, GRP-78, ATF6, CHOP, and caspase-3 expressions in AML12 cells. (d) Individual proteins were quantified. , vs. control group; &, &&& vs. F50+ Sirt-1/Ko group. Ko: gene knockout.
(a) |
(b) |
(c) |
(d) |